Table 2. Pyrosequencing PCR primer sequences and conditions.

ProbeID	Gene	Primers	Tm (°C)
cg10633981	C11orf58	Forward: 5′-TAGAAATAATTGGTGAGGGTAGGA-3′	58
		Reverse: 5′-ACTTCCTAATCTATCAATTCAATATCATAA-3′ – Biotin	58
		Sequencing: 5′-AGGGTTGGGAATTTT-3′	44
cg19933985	IGR	Forward: 5′-TTGGAATTTAGGTGAAGATGTT-3′ – Biotin	56
		Reverse: 5′-CATTAACTAAACCATCATTATACAACC-3′	58
		Sequencing: 5′-ACCATCATTATACAACCT-3′	44
cg11049075	HDAC5	Forward: 5′-TTTTTTAAAGGGATGTGAGTAGGAAGAATT-3′	61
		Reverse: 5′-CCCAAATAACTACAAAAACAAAAATTTACT-3′ – Biotin	59
		Sequencing: 5′-ATTTTTAAATATATAATTAAGATGT-3′	38
cg24449302	MAP2K1	Forward: 5′-GGTATTTGGTTTGGAGAGTTTAT-3′	57
		Reverse: 5′-CTTTCCACACTCCCACTC-3′ – Biotin	57
		Sequencing: 5′-GGATTTAGTTTTTAGGTAGT-3′	39
cg09844907	MPV17L	Forward: 5′-GGGTTTTTTTAGTTTTTAGTAGTTATTGT-3′	57
		Reverse: 5′-CCTTCTACCTCTATAACTTTACCTATTCT-3′ – Biotin	60
		Sequencing: 5′-TGTATTTGTTTTTTTTAGAAGTTT-3′	44
cg17479131	LOC401431	Forward: 5′-GAATTAGAAAGTATATGATGGAGGTTTTAG-3′	58
		Reverse: 5′-ATATAAACCTCTTAAAACAAACTACACT-3′ – Biotin	57
		Sequencing: 5′-AAGAGTATATATGAGTTGATATAG-3′	44

Tm = temperature (annealing).