Table 2.

Illumina Sequence Analysis of Population Genetic Diversity in CVB3 Evolution Lines in which the S₁, P₃, or B₃ Fraction Was Selected after Each Transfer

Virus Population	Total Bases Read	Sites with Mutations	Total Mutant Bases Read	Mutation Frequencya
Founder	1.04 × 108	266	11,254	1.08 × 10−4
Evolved S1, line 1	1.82 × 108	1,868	160,506	8.81 × 10−4
Evolved S1, line 2	1.48 × 108	1,796	150,954	10.20 × 10−4
Evolved P3, line 1	2.43 × 108	2,148	256,770	10.60 × 10−4
Evolved P3, line 2	2.08 × 108	2,092	188,731	9.08 × 10−4
Evolved B3, line 1	1.21 × 108	2,073	89,318	7.38 × 10−4
Evolved B3, line 2	1.99 × 108	2,141	172,507	8.66 × 10−4

^aTotal mutant bases read divided by total bases read.