Figure 3.

Maximum-Likelihood Tree of 631 Identified eCIS Loci (Root on Midpoint)

Based on Afp11 protein sequences, the tree was constructed by FastTree under Whelan Goldman (WAG) models with gamma optimization. Clades with all members from the same species are collapsed for brevity. Loci derived from archaeal and Gram-positive bacterial genomes are highlighted with red and blue branches, respectively. Previously reported eCIS loci are indicated by solid green circles. Outer strips are color coded by taxonomic groups as indicated by the key. The tree scale represents substitutions per site.

See also Figure S2 for details.