Fig. 1.

Time course of the effect of ACTH on Star hnRNA and pCREB in Y1-BS1 and ATC7-L cells. Y1-BS1 (A and C) and ATC7-L (B and D) cells were treated with 10 nM ACTH. Data points represent the mean ± SEM of Star hnRNA (A-B) and pCREB (C-D). Star hnRNA was normalised to Gapdh mRNA and is expressed as fold-changes of time 0 (n = 3–6/time point). In C, pCREB was measured in the nuclear extract, was normalised to HDAC, and is expressed as fold-changes of time 0 (n = 4–5/time point). In D, pCREB was measured in the whole cell extract, was normalised to vinculin, and is expressed as fold-change of time 0 (n = 3/time point). Data in A were analysed using one-way ANOVA followed by Fisher LSD post-hoc test; data in B, C and D were analysed using Welch ANOVA followed by Dunnett T3 post-hoc test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs time 0.