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. 2020 Jan 1;499:110612. doi: 10.1016/j.mce.2019.110612

Fig. 7.

Fig. 7

Effect of inhibition of ACTH signalling on Star hnRNA levels and CRTC activity in ATC7-L cells. Cells were pre-treated for 15 min with either vehicle (0.5%DMSO), protein kinase A inhibitor H89 (H, 10 μM), the MAP kinase inhibitor UO126 (U, 1 μM) or the calcineurin inhibitor CsA (5 μM), either alone or in combination (H89 + UO126 + CsA), prior to treatment with 10 nM ACTH for 10 min. (A) RT-qPCR quantification of Star hnRNA, normalised to Gapdh mRNA and expressed as fold-change levels of the basal vehicle control. Bars represent the mean ± SEM of 3–5 independent experiments. (B) Representative Western immunoblot of nuclear CRTC1, CRTC2 and CRTC3 levels. Quantification of Western immunoblot data of CRTC1 (C), CRTC2 (D), and CRTC3 (E); data are normalised to HDAC and expressed as fold-change of basal. Bars represent the mean ± SEM of 3–5 independent experiments. Data were analysed using two-way ANOVA followed by Fisher LSD post-hoc test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs untreated vehicle control. #P ≤ 0.05, ##P ≤ 0.01, ###P ≤ 0.001 vs ACTH-treated vehicle control.