Antibodies |
|
a-Tubulin |
Thermo Fisher |
A11126 |
CD11b |
Biolegend |
101225 |
CD11c |
Biolegend |
117333 |
Collagen I |
Abcam |
Ab34710 |
Collagen IV |
Abcam |
Ab6588 |
Fibronectin |
Sigma-Aldrich |
F3648 |
F4/80 |
Biolegend |
123125 |
GFP |
BioRad |
47451051 |
I-A/I-E |
Biolegend |
107617 |
Phospho-paxillin |
Cell signaling techology |
2541S |
Podoplanin |
Acris |
DM3501 |
Laminin |
Abcam |
Ab11575 |
LL5beta |
Prof J. Sanes |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
CLEC-2-Fc recombinant protein |
Acton Lab |
https://doi.org/10.1038/nature13814 |
|
Deposited Data |
|
RNaseq data CLEC-2/PDPN signaling in FRCs |
This paper |
https://doi.org/10.5522/04/c.4696979 |
CLEC-2/PDPN signaling phosphoproteomics |
This paper |
https://doi.org/10.5522/04/c.4696979 |
Proteomics of matrix production |
This paper |
https://doi.org/10.5522/04/c.4696979 |
|
Experimental Models: Cell Lines |
|
Immortalized FRCs |
Acton Lab |
https://doi.org/10.1038/nature13814 |
Immortalized FRCs – PDPN KD |
Acton Lab |
https://doi.org/10.1038/nature13814 |
Immortalized FRCs – CLEC-2 expressing |
This paper |
N/A |
|
Experimental Models: Organisms/strains |
|
B6.129S4-Pdgfratm11(EGFP)Sor/J |
Jackson |
007669 |
CD11cΔCLEC-2
|
Francis Crick Institute |
https://doi.org/10.1038/nature13814 |
|
Oligonucleotides |
|
LL5beta siRNA |
Dharmacon |
LQ-062938-01 |
|
Software and Algorithms |
|
STAR aligner software |
Dobin et al., 2013 |
https://github.com/alexdobin/STAR |
Salmon software |
Patro et al., 2017 |
https://combine-lab.github.io/salmon/ |
Bioconductor tximport package |
Soneson et al., 2015 |
https://doi.org/10.18129/B9.bioc.tximport |