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. 2019 Nov 26;29(9):2810–2822.e5. doi: 10.1016/j.celrep.2019.10.103
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

a-Tubulin Thermo Fisher A11126
CD11b Biolegend 101225
CD11c Biolegend 117333
Collagen I Abcam Ab34710
Collagen IV Abcam Ab6588
Fibronectin Sigma-Aldrich F3648
F4/80 Biolegend 123125
GFP BioRad 47451051
I-A/I-E Biolegend 107617
Phospho-paxillin Cell signaling techology 2541S
Podoplanin Acris DM3501
Laminin Abcam Ab11575
LL5beta Prof J. Sanes N/A

Chemicals, Peptides, and Recombinant Proteins

CLEC-2-Fc recombinant protein Acton Lab https://doi.org/10.1038/nature13814

Deposited Data

RNaseq data CLEC-2/PDPN signaling in FRCs This paper https://doi.org/10.5522/04/c.4696979
CLEC-2/PDPN signaling phosphoproteomics This paper https://doi.org/10.5522/04/c.4696979
Proteomics of matrix production This paper https://doi.org/10.5522/04/c.4696979

Experimental Models: Cell Lines

Immortalized FRCs Acton Lab https://doi.org/10.1038/nature13814
Immortalized FRCs – PDPN KD Acton Lab https://doi.org/10.1038/nature13814
Immortalized FRCs – CLEC-2 expressing This paper N/A

Experimental Models: Organisms/strains

B6.129S4-Pdgfratm11(EGFP)Sor/J Jackson 007669
CD11cΔCLEC-2 Francis Crick Institute https://doi.org/10.1038/nature13814

Oligonucleotides

LL5beta siRNA Dharmacon LQ-062938-01

Software and Algorithms

STAR aligner software Dobin et al., 2013 https://github.com/alexdobin/STAR
Salmon software Patro et al., 2017 https://combine-lab.github.io/salmon/
Bioconductor tximport package Soneson et al., 2015 https://doi.org/10.18129/B9.bioc.tximport