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. 2019 Sep 10;28(11):2777–2783.e4. doi: 10.1016/j.celrep.2019.08.026

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Reconstituted CMG Helicase Disassembly with Purified Proteins Requires Ubiquitylation, the Cdc48 ATPase, and Ufd1-Npl4

(A) GAL-ProteinA-TEV-DIA2 PSF2-5FLAG cells (YPM157) were processed as indicated. At the end of the reactions, the GINS component of the CMG helicase was isolated by immunoprecipitation of Sld5, in order to monitor the integrity of CMG.

(B) The samples described in (A) were resolved by SDS-PAGE, and the indicated proteins were analyzed by immunoblotting.

(C) Similar reactions were performed with the indicated factors.

See also Figures S1 and S3.