Table 2.
RNA | Sequence |
---|---|
hsa_GAPDH forward sequence | 5′‐GGGAAACTGTGGCGTGAT‐3′ |
hsa_GAPDH reverse sequence | 5′‐GAGTGGGTGTCGCTGTTGA‐3′ |
hsa_circ_0000745 forward sequence | 5′‐ATGTTGAAAGTAGCCCGAGCAG‐3′ |
hsa_circ_0000745 reverse sequence | 5′‐TGGGAGTGTTGGAAGAAGTTGG‐3′ |
Linear SPECC1 mRNA forward sequence | 5′‐ACCCCAGGAAATCAGTGTCCA‐3′ |
Linear SPECC1 mRNA reverse sequence | 5′‐GTTCCCGAACTTGGGACTCAA‐3′ |
siRNA‐1 | 5′‐CTGGCCAAGGGGCCUUUACTT‐3′ |
siRNA‐2 | 5′‐CUGGCCAAGGGGCCUUUACATT‐3′ |
siRNA‐3 | 5′‐GUCUGCUGGCCAAGGGGCCTT‐3′ |
si‐Negative control | 5′‐UUCUUCGAACGUGUCACGUTT‐3′ |
Note: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; mRNA, messenger RNA; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; siRNA, small interfering RNA.