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. 2019 Jun 29;235(2):1287–1295. doi: 10.1002/jcp.29045

Table 2.

The sequences of primers for qRT‐PCR and siRNAs

RNA Sequence
hsa_GAPDH forward sequence 5′‐GGGAAACTGTGGCGTGAT‐3′
hsa_GAPDH reverse sequence 5′‐GAGTGGGTGTCGCTGTTGA‐3′
hsa_circ_0000745 forward sequence 5′‐ATGTTGAAAGTAGCCCGAGCAG‐3′
hsa_circ_0000745 reverse sequence 5′‐TGGGAGTGTTGGAAGAAGTTGG‐3′
Linear SPECC1 mRNA forward sequence 5′‐ACCCCAGGAAATCAGTGTCCA‐3′
Linear SPECC1 mRNA reverse sequence 5′‐GTTCCCGAACTTGGGACTCAA‐3′
siRNA‐1 5′‐CTGGCCAAGGGGCCUUUACTT‐3′
siRNA‐2 5′‐CUGGCCAAGGGGCCUUUACATT‐3′
siRNA‐3 5′‐GUCUGCUGGCCAAGGGGCCTT‐3′
si‐Negative control 5′‐UUCUUCGAACGUGUCACGUTT‐3′

Note: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; mRNA, messenger RNA; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; siRNA, small interfering RNA.