Figure 1.

With an HRP‐coupled assay, the alcohol oxidation activity of the NOX‐A fusion can be detected without any addition of NADP⁺ (3). The reaction mixture included buffer (50 mm Tris⋅HCl pH 7.5), HRP (0.8 U), AAP (0.1 mm) and DCHBS (1 mm), cell‐free extract containing NOX‐A (10 % v/v) and 30 mm cyclohexanol. Controls: 1) no substrate and 2) no cell‐free extract.