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. 2019 Feb 20;96(6):508–513. doi: 10.1002/cyto.b.21773

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© 2019 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Comparison of volumetric and bead‐based counting of CD34 cells by single‐platform flow cytometry. CD34 cells were enumerated using BD Trucount™ tubes in volumetric cytometers. A CD34 reference material was assessed at two concentrations: CD34 High shown in circles and CD34 Low shown in triangles. The cell concentrations obtained with the number of bead events are shown in the x‐axis and with the recorded acquisition volume in the y‐axis. Outliers are shown in gray. The solid line indicates agreement and the dashed line indicates fitted Deming regression which accounts for error in both variables. The slope is 0.972 with a confidence interval of 0.934–1.02. The intercept is −0.58 with a confidence interval of −1.17 to −0.008.