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. 2019 Jul 11;49(11):2051–2062. doi: 10.1002/eji.201847992

Figure 2.

Figure 2

Effect of statin treatment on the induction of inflammatory Th2 response by TSLP‐stimulated DCs. (A) Naïve CD4+ T cells were cocultured for 7 days with DCs that had been pretreated with medium alone, R848, TSLP, TSLP + 10 µM pitavastatin, TSLP + 10 µM pitavastatin + 100 µM MVA, TSLP + 10 µM simvastatin, or TSLP + 10 µM simvastatin + 100 µM MVA for 24 h. In some cases, anti‐OX40L mAb was added into the DC−T cell cultures. Cytokine production by the primed CD4+ T cells was assessed by measuring the levels in the supernatants. (B) Naïve CD4+ T cells were cocultured for 7 days with DCs that had been pretreated with TSLP, TSLP + 10 µM pitavastatin, and TSLP + 10 µM simvastatin. IL‐9 production by the primed CD4+ T cells was assessed by measuring the levels in the supernatants. (A and B) One set of experiment was performed by DCs from one donor and T cells from another allogenic donor. Data are the mean ± SEM of six (A) and eight (B) independent experiments. Statistical significance was determined using paired Wilcoxon signed‐rank test (*p < 0.05).