Table 1.

Statistically significant features. (a) Index of feature name and identifier used in the study, divided into topological (in white) or nontopological (gray). Topological features are the ones who capture organization, while the nontopological characteristics are morphometric measurements and other nonorganizational quantifications. (b) Results from the univariate analysis performed for tumor genetic instability criteria and high‐risk pretreatment stratification group. Only statistically significant characteristics (χ ² < 0.05) are shown. The features are ranked by their p‐values obtained on the chi‐square test, in ascending order. The selected features to be used in the next steps are underlined. The characteristics of territorial vitronectin (VN) are marked in bold (12/21 in the risk group and 15/27 in tumor genetic instability criteria). Highly statistically significant common features in tumor genetic instability criteria and risk group were marked with an asterisk. MST, minimum spanning tree; std, standard deviation

(a) Definition of features		(b) Univariate analysis
ID	Characteristics	Rank	ID	Characteristics	Chi‐square
Topological		Tumor genetic instability criteria
1	Interterritorial—sorting tensegrity index	1	20	Territorial—Euler number per node*	8.20E‐07
2	Interterritorial—iteration tensegrity index	2	24	Territorial—mean quantity of branches per node*	4.03E‐06
3	Interterritorial—MST tensegrity index	3	37	Territorial—percentage of stained area*	6.58E‐05
4	Interterritorial—std percentage of VN stained area per region	4	16	Interterritorial—std percentage of VN stained area per region	9.05E‐05
5	Interterritorial—std percentage of VN stained area per node	5	39	Total nuclei	1.27E‐04
6	Interterritorial—Euler number per VN stained area	6	29	Territorial—mean percentage of VN stained area per node	1.75E‐04
7	Interterritorial—Euler number per region	7	16	Territorial—std percentage of VN stained area per region	2.74E‐04
8	Interterritorial—Euler number per node	8	45	Territorial—ratio of strong positive pixels to total pixels	2.74E‐04
9	Interterritorial—number of holes per VN stained area	9	27	Territorial—mean percentage of VN stained area per region	3.87E‐04
10	Interterritorial—std area of holes	10	23	Territorial—mean quantity of branches per region	3.87E‐04
11	Interterritorial—mean quantity of branches per region	11	7	Territorial—Euler number per region	5.34E‐04
12	Interterritorial—mean quantity of branches per node	12	40	Percentage of hematoxylin stained nuclei area	6.11E‐04
13	Territorial—sorting tensegrity index	13	22	Territorial—std area of holes	8.44E‐04
14	Territorial—iteration tensegrity index	14	43	Interterritorial—ratio of weak positive pixels to total pixels	1.46E‐03
15	Territorial—MST tensegrity index	15	47	H‐score	0.002
16	Territorial—std percentage of VN stained area per region	16	38	Territorial—VN stained area/mm 2	0.002
17	Territorial—std percentage of VN stained area per node	17	46	Ratio of all positive pixels	0.004
18	Territorial—Euler number per VN stained area	18	31	Territorial—mean area of holes	0.005
19	Territorial—Euler number per region	19	42	Ratio of hematoxylin stained nuclei pixels to total pixels	0.006
20	Territorial—Euler number per node	20	36	Interterritorial—VN stained area/mm2	0.009
21	Territorial—number of holes per VN stained area	21	12	Interterritorial—mean quantity of branches per node	0.014
22	Territorial—std area of holes	22	34	Hematoxylin stained nuclei/mm2	0.016
23	Territorial—mean quantity of branches per region	23	2	Interterritorial—iteration tensegrity index	0.017
24	Territorial—mean quantity of branches per node	24	19	Territorial—Euler number per region	0.024
25	Std difference territorial and Interterritorial	25	21	Territorial—number of holes per VN stained area	0.030
Non‐topological		26	9	Interterritorial—number of holes per VN stained area	0.034
26	Interterritorial—mean percentage of VN stained area per region	27	6	Interterritorial—Euler number per VN stained area	0.045
27	Interterritorial—mean percentage of VN stained area per node	Risk pretreatment stratification group
28	Interterritorial—mean area of holes	1	20	Territorial—Euler number per node*	0.001
29	Territorial—mean percentage of VN stained area per region	2	24	Territorial—mean quantity of branches per node*	0.003
30	Territorial—mean percentage of VN stained area per node	3	39	Total nuclei	0.003
31	Territorial—mean area of holes	4	37	Territorial—percentage of stained area*	0.006
32	Mean difference territorial and Interterritorial	5	16	Territorial—std percentage of VN stained area per region	0.010
33	Percentage of hematoxylin stained nuclei area	6	22	Territorial—std area of holes	0.010
34	Hematoxylin stained nuclei/mm2	7	34	Hematoxylin stained nuclei/mm 2	0.010
35	Interterritorial—percentage of stained area	8	36	Interterritorial—VN stained area/mm 2	0.010
36	Interterritorial—VN stained area/mm2	9	45	Territorial—ratio of strong positive pixels to total pixels	0.010
37	Territorial—percentage of stained area	10	43	Interterritorial—ratio of weak positive pixels to total pixels	0.014
38	Territorial—VN stained area/mm2	11	17	Interterritorial—std percentage of VN stained area per node	0.016
39	Total nuclei	12	29	Territorial—mean percentage of VN stained area per region	0.019
40	Percentage of hematoxylin stained nuclei	13	23	Territorial—mean quantity of branches per region	0.019
41	Percentage of VN positive cells	14	19	Territorial—Euler number per region	0.019
42	Ratio of hematoxylin stained nuclei pixels to total pixels	15	47	H‐score	0.019
43	Interterritorial—ratio of weak positive pixels to total pixels	16	27	Territorial—std percentage of VN stained area per node	0.019
44	Interterritorial—ratio of moderate positive pixels to total pixels	17	38	Territorial—VN stained area/mm 2	0.019
45	Territorial—ratio of strong positive pixels to total pixels	18	33	Percentage of haematoxylin stained nuclei area	0.028
46	Ratio of all positive pixels	19	42	Ratio of haematoxylin stained nuclei pixels to total pixels	0.028
47	H‐score	20	30	Territorial—mean percentage of VN stained area per node	0.033
		21	46	Ratio of all positive pixels	0.033

The asterisk indicated three features that presented low values of chi‐square in both categories.