Figure 3.

APC deficiency reduces the repair of DNA damage induced by a broad spectrum of genotoxic stresses. 1CT and 1CTA cells, grown in 2D culture, were left untreated (see Figure S5) or treated for 6 hr with CDT (1 μg/ml), etoposide (ETOP, 15 μM), camptothecin (CPT, 5 μM), or H₂O₂ (50 μM), defined as 6 hr. After 6‐hr incubation, cells were washed and maintained in medium without the genotoxic agents for additional 18 hr, defined as 24 hr. The percentage of repair was calculated as follows: [1 − (% positive cells at 24 hr/% positive cells at 6 hr)] × 100. One hundred cells were evaluated for each experiment for each cell line. Mean ± SEM of three independent experiments. Statistical analysis was performed using the Student t test, *p < .05; **p < .01; ***p < .001