Figure 6.

APC deficiency promotes acquisition of genomic instability upon infection with the genotoxic Salmonella in a PI3K dependent manner. (a) 1CT and 1CTA cells, grown in 2D culture, were left untreated (Uninf.) or infected with the MC1 TT (TT) strain at MOI 100:1 for the indicated period of time. The left panel shows representative fluorescence micrographs of the infected cells illustrating the presence of micronuclei (white arrowheads) detected by DAPI staining. The right panel shows the quantification of micronuclei positive cells. Mean ± SEM of four to eight independent experiments. (b) 1CTA cells were pretreated for 1 hr with the specific PI3K inhibitor CDG‐0941 (1 μM) for 1‐hr prior infection, which was carried on for 72 hr. DMSO was used as vehicle control. Detection of DNA damage, proliferative capacity, and micronuclei formation were assessed by γH2AX, Ki67, p21, and DAPI staining. Mean ± SEM of three independent experiments. Statistical analysis was performed using the Student t test, *p < .05; **p < .01