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. 2019 Sep 15;58(12):2266–2275. doi: 10.1002/mc.23114

Figure 3.

Figure 3

NCAPH affects HCC cell proliferation. A, NCAPH expression was detected in LM3, Huh7, PLC/PRF/5, and Hep3B cells. B, NCAPH expression were decreased in LM3 and Huh7 cells after transfected shRNAs, and NCAPH expression were increased in PLC/PRF/5 and Hep3B cells after overexpressed NCAPH. C, Knockdown of NCAPH suppressed LM3 and Huh7 cells proliferation by using CCK‐8 assays after 72 hours (P < .001). D, Overexpression of NCAPH promoted PLC/PRF/5 and Hep3B cells proliferation by using CCK‐8 assays after 72 hours (P < .001). E, The results of CFA assays showed that silencing NCAPH expression inhibited LM3 and Huh7 cells proliferation. F, The results of CFA assays showed that overexpressed NCAPH expression promoted PLC/PRF/5 and Hep3B cells proliferation. *Statistical significance. The Student t test was used to compare the differences. CCK‐8, cell counting kit‐8; CFA, colony formation assay; NCAPH, non‐SMC condensin I complex subunit H; shRNA, short hairpin RNA [Color figure can be viewed at wileyonlinelibrary.com]