Figure 1.

Schematic diagram of SUMO variants. Comparison of C‐terminal sequences in mature ubiquitin, wild‐type SUMO‐1, wild‐type SUMO‐2/3, SUMO‐1^Q92R (Q92 mutation to R), SUMO‐1^T95R (T95 mutation to R), SUMO‐2^Q88R (Q88 mutation to R), SUMO‐2^T91R (T91 mutation to R), SUMO‐3^Q87R/Q88N (Q87 and Q88 double mutation to R and N respectively), SUMO‐2^K0T90R (all K replaced with R, meanwhile an additional R is introduced at the AA90), SUMO‐2^K0Q87R (all K in replaced with R, meanwhile a R is introduced at AA87), SUMO‐2^T90K (T90 mutation to K). The length of the tryptic remnant is indicated by the first trypsin‐cleavable residue, and the peptide remnants are highlighted in purple. Similarly, the length of the Lys‐C remnant is indicated by the first Lys‐C‐cleavable residue, and the peptide remnants are highlighted in green. And the introduction of the mutation site is highlighted in red.