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. 2019 Aug 1;50(9):3487–3501. doi: 10.1111/ejn.14513

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© 2019 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies andJohn Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of wild‐type NGF or NGF mutants on proliferation in PC12 cells. Immunofluorescent automatic high‐content image analysis was performed as described in Materials and Methods2. Data show dose‐related effects on number of cell bodies in PC12 cells after treatment with wild‐type NGF (black circles ●), NGF‐R100E (red squares ), NGF‐W99A (green diamonds ) and NGF‐K95A/Q96A (blue triangles ) ranging from 30 pg/ml to 30 ng/ml after 4 days in culture. Seven independent biological repeats with four technical replicates were conducted

Inline graphic — Effects of wild‐type NGF or NGF mutants on proliferation in PC12 cells. Immunofluorescent automatic high‐content image analysis was performed as described in Materials and Methods2. Data show dose‐related effects on number of cell bodies in PC12 cells after treatment with wild‐type NGF (black circles ●), NGF‐R100E (red squares ), NGF‐W99A (green diamonds ) and NGF‐K95A/Q96A (blue triangles ) ranging from 30 pg/ml to 30 ng/ml after 4 days in culture. Seven independent biological repeats with four technical replicates were conducted