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. 2019 Aug 31;100(4):784–800. doi: 10.1111/tpj.14477

Figure 4.

Figure 4

Recovery of the compromised resistance to Verticillium dahliae in VIGS plants by exogenous melatonin pre‐treatment.

(a) Disease symptoms of H2O‐pre‐treated and melatonin‐pre‐treated TRV:00, TRV:SNAT1, TRV:COMT, and TRV:SNAT1 + COMT inoculated with V. dahliae; photographs were taken at 20 dpi. Bar = 1 cm.

(b) Fungal accumulation in the stems of H2O‐pre‐treated and melatonin‐pre‐treated TRV:00, TRV:SNAT1, TRV:COMT, and TRV:SNAT1 + COMT inoculated with V. dahliae at 20 dpi. Bar = 1 cm.

(c) Disease indexes of H2O‐pre‐treated and melatonin‐pre‐treated TRV:00, TRV:SNAT1, TRV:COMT, and TRV:SNAT1 + COMT inoculated with V. dahliae were determined from 10 dpi to 28 dpi. The values are the means ± SD; n = 32.

(d) Relative quantification of fungal biomass. Total DNA from leaves of H2O‐pre‐treated and melatonin‐pre‐treated TRV:00, TRV:SNAT1, TRV:COMT, and TRV:SNAT1 + COMT inoculated with V. dahliae for 20 days was extracted as the template for fungal biomass detection by qPCR. GhUBQ7 was used as the internal control. The values are the means ± SD; n = 3. Statistical analyses were performed using Student's t‐test. *, P < 0.05; **, P < 0.01.