Figure 5.

Freezing tolerance is reduced by 2f‐fucose, a fucose synthesis inhibitor, in a concentration‐dependent manner. Electrolyte leakage values from Col‐0 wild‐type (WT) Arabidopsis thaliana plants treated with DMSO and three different concentrations of 2f‐fucose. Plants were grown on MS agar containing DMSO, 2.5 µM, 10 µM or 25 µM of 2f‐fucose for 2 wk. Values represent percentage loss of electrolytes from seedlings when exposed to temperatures of −3, −5 and −7°C. Each data point represents the average of three separate biological replicate experiments. Each experiment used six replicate tubes per treatment per temperature, with c. 10 mg seedlings per tube. Arcsine‐transformed percentage leakage data were analysed by a least‐squares means comparison at each temperature point (*, P < 0.05; ***, P < 0.001). Error bars represent ± 1 SE of arcsine‐transformed data.