Figure 5.

Semisynthetic strategies for prion protein (PrP) variants, developed in the Becker laboratory.228, 255, 256 Via an expressed protein ligation (EPL)‐ (A) and a protein trans‐splicing (PTS)‐based (B) approach, PrP variants equipped with a glycosylphosphatidylinositol (GPI) anchor mimic can be obtained. With the strategy displayed in C, PrP variants modified with monodisperse PEG chains as mimics of N‐glycans can be accessed. Selective desulfurization of the introduced cysteine following EPL (A) is depicted in D