Table 1.

TGFBR1 Target Sequences With Protospacer Adjacent Motif (PAM) and Primer Sequences For Targeted Deep Sequencing

Names	Sequence
TGFbR1 sgRNA target #5	TAAAAGGGCGATCTAATGAAGGG
TGFbR1 sgRNA target #1	GAACTGGCAGCTGTCATTGCTGG
TGFbR1 sgRNA target #2	GAGATGCAGACGAAGCACACTGG
TGFbR1 sgRNA target #3	CCATCGAGTGCCAAATGAAGAGG
TGFbR1 sgRNA target #4	CCTCTTCATTTGGCACTCGATGG
TGFbR1 sgRNA target #6	AGATCGCCCTTTTATTTCAGAGG
TGFbR1 sgRNA target #7	GATCGCCCTTTTATTTCAGAGGG
First primer_forward	TTATTTCACTCGAGGCCCTTTTTCAGTAAA
First primer_reverse	GGGTTAGCTGCAGATCATGTGAATATC
Deep seq primer_forward	TTATTTCACTCGAGGCCCTTTTTCAGTAAA
Deep seq primer_reverse	CAGAACCTGACGTTGTCATATCATAAATTA