Table 1.
Primer sequences used for real‐time quantitative polymerase chain reaction
| Gene name | Gene symbol | Primers (5′‐3′) |
|---|---|---|
| Fibronectin | Fn | Forward: TAGCCCTGTCCAGGAGTTCA |
| Reverse: CTGCAAGCCTTCAATAGTCA | ||
| Collagen 1 | Col1 | Forward: GCAGGAGGTTTCGGCTAAGT |
| Reverse: GCAACAAAGTCCGCGTATCC | ||
| Transforming growth factor‐β1 | Tgfβ1 | Forward: CCACCTGCAAGACCATCGAC |
| Reverse: CTGGCGAGCCTTAGTTTGGAC | ||
| Tumor necrosis factor‐α | Tnfα | Forward: CATCTTCTCAAAATTCGAGTGACAA |
| Reverse: TGGGAGTAGACAAGGTACAACCC | ||
| Interlekin‐1β | Il‐1β | Forward: GTCTTTCCCGTGACCTTC |
| Reverse: ATCTCGGAGCCTGTTAGTGC | ||
| Uncoupling protein 1 | Ucp1 | Forward: AGGCTTCCAGTACCATTAGGT |
| Reverse: CTGAGTGAGGCAAAGCTGATTT | ||
| Peroxisome proliferator‐activated receptor‐γ coactivator‐1α | Pgc1α | Forward: CCCTGCCATTGTTAAGACC |
| Reverse: TGCTGCTGTTCCTGTTTTC | ||
| Cd36 | Cd36 | Forward: TGTGTTTGGAGGCATTCTCA |
| Reverse: TTTTGCACGTCAAAGATCCA | ||
| Adipose triglyceride lipase | Atgl | Forward: TGTGGCCTCATTCCTCCTAC |
| Reverse: TCGTGGATGTTGGTGGAGCT | ||
| Cytochrome c oxidase subunit 2 | Cox2 | Forward: CCATCCCAGGCCGACTAA |
| Reverse: AATTTCAGAGCATTGGCCATAGA | ||
| Klotho beta | Klb | Forward: CAGGCCCATTGTTACCTTGT |
| Reverse: CTCCAAAGGTCTGGAAGCAG | ||
| Fibroblast growth factor receptor 1c | Fgfr1c | Forward: AATACCACCGACAAGGAAATGG |
| Reverse: AGTTACCCGCCAAGCACGTA | ||
| 18S | Forward: AGCCTGCGGCTTAATTTGAC | |
| Reverse: CAACTAAGAACGGCCATGCA |