Figure 2.

Mtb FadD13 promotes production of proinflammatory cytokines in macrophages. (a) The differentiated THP‐1 macrophages were infected at a multiplicity of infection (MOI) of 10 with M. smegmatis containing the empty vector (pMV261 without H37Rv FadD13 DNA) or pMV261‐FadD13 respectively. (b) The differentiated THP‐1 macrophages were infected at a MOI of 10 with ΔfadD13 mutant and H37Rv respectively. Enzyme‐linked immunosorbent assay (ELISA) was used to detect the production of IL‐1β, IL‐18, and IL‐6 in the supernatant in both (a) and (b). (c) The differentiated THP‐1 macrophages were infected at a MOI of 10 with ΔfadD13 mutant and H37Rv respectively. ELISA was used to detect the production of IL‐10 in the supernatant. The results presented as the one replicate from three independent experiments. ^* p < .05 and ^** p < .01 (two‐tailed unpaired t test)