Skip to main content
. 2019 Aug 26;37(12):2540–2549. doi: 10.1002/jor.24430

Figure 1.

Figure 1

Cannabidiol (CBD) reduces viability and induces apoptosis in human chondrocytes. Relative viability of C28/I2 cells (A) or human primary chondrocytes (B) treated with 3–30 µM CBD for 2 and 24 h. Results are given as % of untreated controls (UTC). Mean ± standard deviation (SD) of three independent 7‐hydroxy‐3H‐phenoxazin‐3‐one‐10‐oxide sodium salt (Resazurin) experiments. (C) Flow cytometry analysis of annexin‐V/7‐actinomycin D (7‐AAD) staining in C28/I2 cells treated ± CBD. (D) Caspase 3/7 activity (% of UTC) of C28/I2 cells treated with rising concentrations of CBD. Mean ± SD of three independent experiments. (E) Microscopy images of C28/I2 cells untreated, starved, or starved and treated with 10 or 30 µM CBD for 2 h.