Skip to main content
. 2019 Oct 17;58(48):17351–17358. doi: 10.1002/anie.201907901

Figure 5.

Figure 5

A) SDS PAGE gel of competition pull‐down. Bead‐immobilized biotinylated PBM incubated with NF‐YB/C (80 μm) in the presence or absence of 2‐DN or 2‐D (c=16, 80, 400 μm; for triplicate and full gels, see Figure S20). B) Fraction of bound DNA as determined by FP using TAMRA‐labeled DNA (c=5 nm) with NF‐YA(262–332) (c=25 nm) and NF‐YB/C (c=4 nm) in the absence and presence of peptide (c=10 μm, triplicate measurements, errors account for 1σ). ns: p>0.05, ** p<0.01, *** p<0.001. C) FP titration using TAMRA‐labeled DNA (c=5 nm) and varying concentrations of NF‐Y trimer (c=9.7×10−11−3.7×10−7  m) in the absence or presence of peptide (c=10 μm).