Skip to main content
. 2019 Sep 9;100(4):738–753. doi: 10.1111/tpj.14467

Figure 2.

Figure 2

Time‐course analysis of developing sun and shade leaves in the wild type. (a) Longitudinal sections of developing leaf blades in shade leaves (upper: low light) and sun leaves (lower: high light) sampled at 4, 5, 6, 7, 8 and 10 days after sowing (DAS). For morphological observations, images of optical sections were taken under a confocal laser microscope (4–8 DAS) or of Technovit sections under a light microscope (10 DAS). Typical mesophyll tissue cells are outlined at each stage, and changes in cell layers of leaf primordia at 4 and 10 DAS are shown. We analysed three samples per condition, and the most representative data are shown. (b) Ploidy levels of developing sun and shade leaves at 6, 7, 8, 10 and 14 DAS. Samples contained more than 10 first foliage leaves (from five individual plants) at 6–8 DAS or six first foliage leaves (from three plants) at 10–14 DAS. (c) Leaf length, (d) leaf petiole length, (e) leaf blade area and (f) leaf blade length in developing leaves at 5–10 DAS. Values represent means ± SDs (n = 10 leaves from five plants). Asterisks indicate statistically significant differences by Student's t‐test (P < 0.0001). Scale bars: 50 μm.