Table 3.

Conservation of the human ACTN1 residues Thr257, Val328, Gln329, Lys324, Lys398, Ala432, Arg450, His622, Gln719, and Met748 in orthologs

Species		UniProt ID	Residues										Identity rate (%)
													ACTN1	Rod domain
Human	Homo sapiens	P12814	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	Reference	Reference
Chimpanzee	Pan troglodytes	H2RDW7	T244	V315	K311	Q316	K385	A419	R437	H609	Q706	M735	99.8	100.0
Macaque	Macaca mulatta	H9YUP4	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	99.9	100.0
Dog	Canis lupus familiaris	E2QY08	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	99.3	99.3
Cow	Bos taurus	Q3B7N2	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	99.4	99.3
Mouse	Mus musculus	Q7TPR4	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	99.2	99.3
Rat	Rattus norvegicus	Q6T487	T257	V328	K324	Q329	K398	A432	R450	H622	Q719	M748	99.3	99.3
Zebrafish	Danio rerio	B8JHU4	T267	V338	K334	Q339	K408	A442	R460	H632	Q729	M758	93.4	91.9
Xenopus	Xenopus tropicalis	F7B6G0	T255	V326	K322	Q327	K396	A430	R448	H620	Q717	M746	94.9	93.2

Note: The amino‐acid sequence of human ACTN1 protein was compared to orthologs using Clustal OMEGA (http://www.ebi.ac.uk/Tools/msa/clustalo/). The novel variations reported in this study alter the residues Thr257, Val328, Lys324, Gln329, Lys398, Ala432, Arg450, His622, Gln719, and Met748. The table gives the corresponding residue in eight other species. Identity rate is provided for the entire ACTN1 sequence (penultimate column) and for the rod domain sequence (last column), showing that ACTN1 sequence and its rod domain are highly conserved between these species.