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. 2019 Sep 29;112(5):1609–1625. doi: 10.1111/mmi.14386

Figure 2.

Figure 2

MarA activates transcription by σ70 but not σ38‐associated RNA polymerase at the ycgZ‐ymgABC promoter.

A. Activation of the ycgZ‐ymgABC promoter requires MarA side chain W19. The figure shows in vitro transcription assays using the ycgZ.1 DNA fragment cloned in plasmid pSR as a template. RNA polymerase was used at a concentration of 0.4 μM and MarA was used at concentrations of 0.4, 1.2, or 2.0 μM. Transcripts generated from the ycgZ‐ymgABC promoter are labelled and the RNAI transcript is generated from the plasmid replication origin.

B. Activation of the ycgZ‐ymgABC promoter requires the marbox. The figures show results of in vitro transcription assays. The DNA template was plasmid pSR carrying the ycgZ.1, ycgZ.1m or ycgZ.2 DNA fragments. RNA polymerase was used at a concentration of 0.4 μM and MarA was used at concentrations of 0.4, 1.2 or 2.0 μM.