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. 2019 Sep 29;112(5):1609–1625. doi: 10.1111/mmi.14386

Figure 3.

Figure 3

Spacing and orientation requirements for activation of the ycgZ‐ymgABC promoter by MarA.

A. The schematics show the ycgZ.1 DNA fragment and derivatives. The marbox is shown as a green arrow to depict orientation. The centre of the marbox with respect to the transcription start site (black bent arrow) is indicated. Promoter elements are in grey and labelled. Note that the UP‐element half‐site is labelled ‘UP’ for brevity.

B. Activation of transcription from different derivatives of ycgZ.1 by σ70‐associated RNA polymerase. The data are images of gels used to separate products from in vitro transcription assays. The DNA template was plasmid pSR carrying the different ycgZ.1 derivatives. RNA polymerase was used at a concentration of 0.4 μM and MarA was used at concentrations of 0.4, 0.8, 1.2, or 2.0 μM.

C. Activation of transcription from different derivatives of ycgZ.1 by σ38‐associated RNA polymerase. Data are otherwise as described for panel B. [Colour figure can be viewed at https://www.wileyonlinelibrary.com]