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. 2019 Sep 3;78(12):1621–1631. doi: 10.1136/annrheumdis-2018-214967

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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The monoclonal ACPA clones 1325:01B09 and 1325:04C03 bind to synovial targets in patients with RA but not in healthy controls. The immunohistochemistry images illustrate binding patterns of the monoclonal ACPA clones 1325:01B09 and 1325:04C03 to synovial tissues obtained from healthy donors (A) or patients with RA (B). Mouse IgG1 and the non-citrulline-specific 1362:01E02 (E02) monoclonal antibody were used as controls. CD55 and podoplanin (PDPN) stainings highlight FLS-rich areas of the synovial membrane. Immunohistochemistry stainings were analysed with light microscopy using 250x original magnification. (C) Confocal microscopy images illustrate a partial colocalisation of the ACPA clone 1325:01B09 with CD55 (C) and PDPN (D), highlighted by closed arrows. Stainings with the monoclonal ACPA 1325:04C03 and the control 1362:01E02 antibodies are also shown. Moreover, partially colocalisation of 1325:01B09 F(ab’)2 with PDPN were highlighted by arrows (E). An original magnification of 400x was used for confocal microscopy. ACPA, anticitrullinated protein/peptide antibody; DAPI, 4,6-diamidino-2-phenylindole; FLS, fibroblast-like synoviocytes; RA, rheumatoid arthritis.