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. 2019 Dec 4;11:10229–10238. doi: 10.2147/CMAR.S221150

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© 2019 Sun et al.

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Isolation and identification of colorectal cancer (CRC) stem cells in the SW620 cell line and determination of miR-194 and slingshot 2 (SSH2) expression levels in CRC stem cells and non-stem cells. (A) The proportions of CD44+ and CD133+ cells were analyzed by flow cytometry before and after isolation with microbeads. Before isolation, CD44+/CD133+ cells accounted for 4.53% of the total number of cells. After isolation, CD44+/CD133+ cells accounted for 88.7% of the total number of cells. (B) CRC stem cells were cultured in serum-free medium and sphere formation was evaluated under a microscope after 10 days. Scale bar = 100 µm. (C) RT-qPCR results showed that the expression levels of miR-194 were reduced (*P<0.05 according to the two-tailed t test). (D)The expression levels of SSH2 were increased in CRC stem cells compared with those in CRC non-stem cells (*P<0.05 according to the two-tailed t test). (E) The SSH2 protein expression levels were increased in CRC stem cells compared with those in CRC non-stem cells.