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. Author manuscript; available in PMC: 2020 May 25.
Published in final edited form as: Nat Struct Mol Biol. 2019 Nov 25;26(12):1132–1140. doi: 10.1038/s41594-019-0331-x

Extended Data Fig. 2. Cryo-EM analysis of ribosomes stalled on poly(A).

Extended Data Fig. 2

(A) Representative micrograph of poly(A)-stalled ribosomes used for single particle analysis. (B) Data processing scheme used for structure determination in Relion 3.0. 3D classification reveals that ~90% of active ribosomes are in the canonical state with P/P tRNA while ~10% are seen in the rotated state with A/P and P/E hybrid state tRNAs. The majority of the rotated state ribosomes also contain density for a preceding ribosome and therefore represent ribosomes that have collided with a poly(A)-stalled ribosome. (C) Fourier shell correlation (FSC) curve of the final map illustrating an overall resolution of 2.8 Å.