Table 1. Cryo-EM data collection, refinement and validation statistics.
| Poly(A)-stalled ribosome (EMD-10181, PDB 6SGC) |
|
|---|---|
| Data collection and processing | |
| Magnification | 75,000 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 41.8 |
| Defocus range (μm) | -1.5 to -2.7 |
| Pixel size (Å) | 1.085 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 673,452 |
| Final particle images (no.) | 148,615 |
| Map resolution (Å) | 2.80 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.4-3.5 |
| Refinement | |
| Initial model used (PDB code) | 5LZS |
| Model resolution (Å) | 2.80 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 2.4-3.5 |
| Map sharpening B factor (Å2) | -20 |
| Model composition | |
| Nonhydrogen atoms | 219,039 |
| Protein residues | 11,945 |
| Nucleic acid residues | 5,730 |
| Metals (Mg2+/Zn2+) | 275/8 |
| Ligands | 2 |
| B factors (Å2) | |
| Protein | 49.9 |
| Nucleotide | 64.5 |
| Ligand | 29.2 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.003 |
| Bond angles (°) | 0.500 |
| Validation | |
| MolProbity score | 1.65 |
| Clashscore | 7.01 |
| Poor rotamers (%) | 0.01 |
| Ramachandran plot | |
| Favored (%) | 96.15 |
| Allowed (%) | 3.78 |
| Disallowed (%) | 0.07 |