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. 2019 Dec 4;14:9547–9561. doi: 10.2147/IJN.S225172

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© 2019 Samaddar et al.

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Effect of N/P ratio on polyplex properties. (A) Hydrodynamic diameter (number mean); (B) polydispersity; (C) zeta potential; (D) gel-shift assay for polyplex prepared by mixing 60 µg/mL plasmid with CD_2.5kD-PEI in 20mM HEPES buffer at different N/P ratios. The total flow rate was 10 mL/min and the flow rate ratio was 1:1. Results are the mean ± SD (n=3).