Figure 2.

Knockdown of circ‐TSPAN4 inhibits LUAD metastasis. (a) qRT‐PCR analysis for circ‐TSPAN4 expression in stably circ‐TSPAN4‐depleted H1975 and H1993 cell lines. (b‐d) Cell wound scratch and transwell assays in stably circ‐TSPAN4‐depleted H1975 and H1993 cells. (e, f) Lung metastasis model was established by tail vein injection of circ‐TSPAN4‐depleted H1993 cells, the lung metastasis was monitored by the IVIS Lumina II system and Haematoxylin‐eosin staining. * p < .05. All data are presented as mean ± SD of at least three independent experiments