Skip to main content
. 2019 Feb 12;39(2):BSR20181122. doi: 10.1042/BSR20181122

Figure 4. IGF-1 inhibition of HD-induced mitochondrial apoptosis via the PI3K/Akt signaling pathway in the VSC4.1 cells.

Figure 4

VSC4.1 cells were exposed to 10 mM 2,5-HD, 10 mM 2,5-HD + 50 ng/ml IGF-1 for 24 h. Western blot analysis was used to detect Akt, p-Akt, Bad and p-Bad protein expression. (A and B) Detection of expression levels of Akt and p-Akt (A) or Bad and p-Bad (B), respectively. Data are presented as mean ± S.D. (n=3). (C) Examination of the dimerization of Bad/Bcl-xL complex in the mitochondria using Co-IP. (D) Immunofluorescence test for Cyt c release in VSC4.1 cells. (E) The detection of caspase-3 activity. (F) TUNEL labeling with DAPI counter staining was performed to detect apoptotic cells in VSC4.1 cells. (G) Quantification of the abundance ratio of TUNEL-positive cells in each condition. aP<0.05, compared with control group results; bP<0.05, compared with the results of the group exposed to 10 mM 2,5-HD.