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U251 cells and C6 cells were pretreated with CQ (15 μM) for 1 h before being exposed to 15 μM IVM. The percentage of apoptotic cell was evaluated by flow cytometry after cells were incubated with IVM (15 μM) in the presence or absence of CQ (15 μM). Q2 plus Q4 areas were calculated as the apoptosis ratio. Data were presented as the means ± SD of three independent tests. **P<0.01, ***P<0.001, as compared with IVM group.
