FIGURE 3.
The t1/2 circulating in blood of mAb 3d-8b is decreased in C3-sufficient animals. C3−/− mice (C3 KO [knockout], n = 3) and wild-type (wt) B6 mice (n = 3) were injected with 1.5 mg of mAb 3d-8b. Similarly, two groups of mice were injected with isotype control IgG2b (anti-OVA mAb). Mice were bled at 22 h and 9 and 18 d later. The level of mAb 3d-8b was measured in ELISA with recombinant mC3d as the substrate. Control Ab detection was done using an OVA ELISA. The level of mAb 3d-8b did not decrease quickly at early timepoints (22 h); however, with time, the Ab was eliminated to a very low level (2 μg/ml) at day 18. Elimination was C3 dependent, as the C3 KO mice demonstrated different kinetics of mAb 3d-8b reduction in the blood. The kinetics of 3d-8b elimination from C3 KO mice was similar to that observed for isotype control mAb.