FIGURE 4.

Single injection of mAb 3d-8b results in a long-lasting ameliorative effect on autoantibody production and proteinuria. (A) Thirteen-week-old MRL/lpr mice were divided into three groups with 12 mice in each group and injected with 2 mg/mouse mAb 3d-8b (circle), 2 mg/mouse of isotype control mAb (square), or PBS alone (triangle). Each symbol represents data for an individual mouse. The horizontal line is the average for each group. The level of anti-dsDNA IgG Ab in plasma, presented in R.U., shows that mice injected with mAb 3d-8b demonstrate a dramatic reduction in dsDNA Ab production. (B) Urine samples were obtained from the same mice and analyzed for the level of proteinuria. For that, the level of albumin was normalized using the creatine level in the same urine sample. The level of proteinuria was also substantially reduced in mice injected with mAb 3d-8b. Included is a representative experiment from three independent studies. Statistical analysis was performed by one-way ANOVA with adjustment for multiple comparisons using the Dunnett–Hsu test. *p < 0.05. ns, not statistically significant.