FIGURE 8.

Effect of 3d-8b mAb injection on splenic B cell subpopulations in addition to T and B cell populations in spleen. (A) Seven- and fourteen-week-old MRL/lpr mice (three per group) were injected with 2 mg/mouse of mAb 3d-8b, isotype control, or PBS alone. One week later, spleens were taken, and cells were stained with CD19–allophycocyanin, CD23–PE, or CD24–FITC. Histogram represents gating stratagy for CD19⁺ cells. CD19⁺ cells were analyzed for levels of expression for CD24 and CD23 markers. Percentage of follicular (CD23⁺, CD24^lo), MZ (CD23⁻, CD24^lo), transitional (CD23⁺, CD24^hi), and immature (CD23⁻,CD24^hi) cells on gated CD19⁺ cells was calculated. Bars represent mean percentage data calculated for each mouse in particular group. Stars show the differences in MZ cells between young and old MRL/lpr mice. Error bars represent SD for a particular group of data. (B) Splenic cells from mice described in (A) were stained with anti-CD4–allophycocyanin, anti-CD8–FITC and anti-CD19–PE Ab. The average percentage of CD8, CD4, and CD19-positive spleen cells in total cell population is presented in the bar graph. Error bars represent SD for a particular group of data. Statistical analyses were performed by one-way ANOVA, comparing data obtained for mAb 3d-8b–injected mice to control Ab– or PBS-injected mice. ns, not statistically significant.