Table 2.
Plasmids used or constructed in this study.
| Name | Description | Source |
|---|---|---|
| pET28a | Kan R, ori ColE1, Ptac | Novagen |
| pZA33 | CmR, ori p15A, Pa1lac0−1 | Expressys |
| pZA27 | Kan R, ori p15A, proC | This study |
| pZA36 | Cm R, ori p15A, Ptac | This study |
| pZA38 | Cm R, ori p15A, proC | This study |
| pZS23 | Kan R, ori pSC101, Pa1lac0−1 | Expressys |
| pZS27 | Kan R, ori pSC101, proD | This study |
| pA4 | pZS23 aldA | This study |
| pA7 | pZS27 aldA | This study |
| pKF3 | pZA36 kdsD fsaA | This study |
| pKF6 | pZS38 aldA | This study |
| pVT-FSAA | pET28 fsaA | This study |
| pVT-KDSD | pET28 kdsD | This study |
| pVT-ALDA | pET28 aldA | This study |