Figure 4.
DCPIB alters mitochondrial respiration in HEK‐293 cells. (a) Gene expression of LRRC8A in WT and LRRC8A KO cells (n = 4). (b) Currents were measured by stepping Vm from −200 mV to +200 mV in 20‐mV increments. Cells were exposed to isotonic bath, hypotonic bath, or hypotonic bath +10 µM DCPIB to inhibit VRAC. Mitochondrial respiration in presence of vehicle (circles) or DCPIB (10 µM, squares) in (c) WT and (d) LRRC8A‐KO HEK cells. Mitochondrial respiration was measured by sequential addition of vehicle/DCPIB (10 µM), oligomycin (1 µM), FCCP (1 µM) and rotenone & antimycin A (0.5 µM each). O, oligomycin; F, FCCP; R/AA, rotenone/antimycin A