Table 2.
Results of three-way permutational multivariate analyses of variance conducted on photo-physiological variables (photosynthetic parameters and pigment content), and multivariate gene-expression data (-ΔCT values). P (perm) < 0.05 are in bold.
Three-Way Permutational Multivariate Analysis of Variance | |||||
Source | df | Pseudo-F | P (perm) | Unique perms | Pair-wise tests |
Photo-physiology | |||||
Heat | 1 | 196.23 | 0.000 | 9851 | |
Leaf Rank (LR) | 2 | 12.406 | 0.000 | 9958 | LR: 1 ≠ 2 = 3 |
Leaf Height (LH) | 2 | 18.242 | 0.000 | 9957 | LH: B = M ≠ H |
Heat × LR | 2 | 1.421 | 0.257 | 9950 | |
Heat × LH | 2 | 6.281 | 0.004 | 9960 | B: Control ≠ Heated; M: Control ≠ Heated; H: Control ≠ Heated |
LR × LH | 4 | 1.393 | 0.266 | 9957 | |
Heat × LR × LH | 4 | 0.475 | 0.756 | 9948 | |
GOIs | |||||
Heat | 1 | 81.799 | 0.000 | 9945 | |
Leaf Rank | 2 | 0.467 | 0.753 | 9959 | |
Leaf Height | 2 | 3.168 | 0.019 | 9941 | LH: B ≠ H; M = B, H |
Heat×LR | 2 | 0.986 | 0.392 | 9954 | |
Heat×LH | 2 | 0.510 | 0.717 | 9946 | |
LR×LH | 4 | 0.662 | 0.699 | 9940 | |
Heat×LR×LH | 4 | 0.779 | 0.595 | 9938 |