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. 2019 Nov 19;116(49):24786–24795. doi: 10.1073/pnas.1916331116

Fig. 3.

Fig. 3.

Assembly of the export apparatus results in pronounced membrane remodeling. (A) A vertical section from in situ cryo-ET structure of the injectisome from a ∆invA S. Typhimurium strain (the dotted box denotes area of detail shown in B–G). (B–G) Vertical sections of cryo-EM (B–D) and cryo-ET (E–G) structures of the inner rings of the needle complex T3SS complex overlaid with a section (B and E), surface rendering (C and F), or hydrophobicity surface representation (D and G) of the FliP/FliQ/FliR (homologs of SpaP/SpaQ/SpaR from S. Typhimurium) isolated complex (PDB ID code 6F2E). Red and blue colors (D and G) on the surface representation of the FliP/FliQ/FliR isolated complex represent hydrophobic and charge residues, respectively. IM, inner membrane; IR1, inner ring 1; OM, bacterial outer membrane.