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Evaluation of fluorescent nonapeptide substrates at appropriate enzyme concentration. Fluorescence enhancement velocity of 20 μmol/L fluorescent nonapeptide substrate (ne-T9, ne-H9, on-H9, ne-P9, ne-K9, or ne-D9) incubating with different SIRTs [1 μmol/L SIRT1(A), 0.3 μmol/L SIRT2(B), 0.4 μmol/L SIRT3(C), or 2 μmol/L SIRT6(D)], and 1 mmol/L NAD+ in assay buffer at 37 °C for 30 min. Data are presented as mean±SD, n=3; ∗P<0.05 vs. ne-T9.
