Figure 5.

Dose–response curves and IC₅₀ value for AGK2 and SirReal2 examined by ne-D9-based assay and ne-K4a-based assay. Dose–response inhibition experiments for AGK2 and SirReal2 in (A) ne-K4a-based assay and (B) ne-D9-based assay. The IC₅₀ values of AGK2 and SirReal2 were determined to be 20.02±2.47 μmol/L and 0.96±0.56 μmol/L, respectively, in ne-K4a-based assay. The IC₅₀ value of AGK2 was determined to be 10.71±3.88 μmol/L. The reactions were performed with 20 μmol/L ne-K4a or ne-D9, 1 mmol/L NAD⁺, 0.3 μmol/L SIRT2 and inhibitor (AGK2 or SiReal2) at gradient concentrations (0, 0.2, 1, 6, 30, 100, and 500 μmol/L) in assay buffer at 37 °C for 30 min. Data are presented as mean±SD, n=3.