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Evaluation of newly developed fluorescent substrates, ne-D9-S and ne-d9-M. (A) The structures of two developed ne-D9 fluorescent substrates, ne-D9-S and ne-D9-M. (B) Fluorescence enhancement velocity of 20 μmol/L of substrates (ne-D9-S and ne-D9-M) incubating with SIRT2 at gradient concentrations (0.3, 1.5 and 3 μmol/L), 1 mmol/L NAD+ in assay buffer at 37 °C for 30 min. Data are presented as mean±SD, n=3.
