Table 1.
Yeast and human mRNA 3′-end processing machinery
| Yeast complex | Module | Yeast proteina | M.W. (kDa) | Human Proteina | M.W. (kDa) |
Human complex | % Sequence identity (similarity) | Proposed role |
|---|---|---|---|---|---|---|---|---|
| CPF | Polymeraseb | Pap1 | 65 | PAP | 83 | 40 (59) | Poly(A) polymerase | |
| Cft1/Yhh1 | 153 | CPSF160/CPSF1 | 161 | CPSF | 17 (34) | Scaffold | ||
| Pfs2 | 53 | WDR33 | 146 | 37 (57) | Scaffold, RNA binding | |||
| Fip1 | 36 | hFip1 | 67 | 22 (34) | Binds Pap1 | |||
| Yth1 | 25 | CPSF30/CPSF4 | 30 | 36 (51) | RNA binding | |||
| Nucleaseb | Cft2/Ydh1 | 96 | CPSF100/CPSF2 | 88 | 22 (41) | (Pseudo-)endonuclease | ||
| Ysh1/Brr5 | 88 | CPSF73/CPSF3 | 77 | 44 (65) | Endonuclease | |||
| Mpe1 | 50 | RBBP6 | 202 | 28 (48) | RNA binding | |||
| Phosphatase | Pta1 | 89 | Symplekin | 141 | 17 (37) | Scaffold | ||
| Ref2 | 60 | n.d. | – | – | Regulates Glc7 | |||
| Pti1 | 47 | n.d. | – | – | Scaffold | |||
| Swd2/Cps35 | 37 | WDR82 | 35 | 34 (52) | Transcription termination | |||
| Glc7 | 36 | PP1A | 38 | 85 (93) | Phosphatase of Pol II CTD | |||
| Ssu72 | 24 | SSU72 | 23 | 44 (65) | Phosphatase of Pol II CTD | |||
| CF IA | Rna14 | 78 | CstF77/CSTF3 | 83 | CstF | 26 (42) | Stoichiometry of 2, scaffold | |
| Rna15 | 33 | CstF64/CSTF2 | 61 | 27 (41) | Stoichiometry of 2, RNA binding | |||
| n.d. | CstF50/CSTF1 | 48 | – | |||||
| Clp1 | 50 | hClp1 | 48 | CF IIm | 27 (44) | RNA kinase in human | ||
| Pcf11 | 72 | hPcf11 | 173 | 21 (32) | Binds Pol II | |||
| CF IB | Hrp1 | 60 | n.d. | – | – | RNA binding | ||
| n.d. | CFIm25/CPSF5/NUDT21 | 26 | CF Im | – | RNA binding | |||
| n.d. | CFIm68/CPSF6 | 59 | – | RNA binding | ||||
| n.d. | CFIm59/CPSF7 | 52 | – | RNA binding |
n.d., none detected.
a
Enzymes in bold.
b
Yeast polymerase and nuclease modules comprise the ‘Core CPF’ (CPFcore).