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. 2019 Nov 19;116(49):24630–24638. doi: 10.1073/pnas.1913992116

Fig. 1.

Fig. 1.

Measuring the synthesis of 2 replicons during EBV’s lytic phase. (A) The 2 replicons in iD98HR1-4012 cells are depicted. P3HR1 is a strain of EBV derived from the P3HR1 cell line and is 165 kb in length; 4012, a derivative of EBV, is 36 kb. The cis-acting elements essential for these studies are depicted and include oriP (latent origin of replication), oriLyt (the lytic origin of DNA synthesis), TR (the terminal repeats required for encapsidation of synthesized DNA), and LacO (the site to which the Lac repressor binds). (B) iD98HR1-4012 cells were induced to support the lytic phase by treatment with tamoxifen. The immediate early gene BZLF1 fused to the estrogen receptor is expressed constitutively in these cells. DNA was isolated from these cells at the indicated times and assayed for the amount of viral DNA and cellular DNA using qPCR (for time points 0, 20, 40, and 60 hpi, n [biological replicates] = 4; for 11, 33, and 36 hpi, n = 2). The error bars indicate the SD of the mean of the levels of EBV DNA/cell (with the assumption that each cell contains 2 copies of the rhodopsin gene).